How To: Plant Tissue Planlet Care

Planlet Incubation

Keep the plantlet in the container provided by us, then transfer it into a carefully controlled incubation environment for the shoot induction phase.

Incubate the culture: Place the culture in an incubator room with the following conditions:

• Temperature: Around 24-26°C (75-79°F).
• Light: A 16-hour light/8-hour dark cycle.
• Humidity: Around 70-80%.

Check for shoot development: Over the next 9 weeks, regularly check for the development of shoots. Transfer to greenhouse when roots are established.

Greenhouse Care

Materials Needed:

• Greenhouse or similar controlled environment
• Pots
• Potting soil or a soil-less medium

Procedure:

Prepare for acclimatization: Fill pots with potting soil or a soil-less medium and carefully remove the plantlets from the culture medium, taking care not to damage the roots. Rinse off any remaining medium.

Plant the plantlets: Place the plantlets in the prepared pots.

Begin acclimatization: Start with high humidity (around 80-90%) and low light intensity in a controlled greenhouse environment, then gradually decrease humidity and increase light intensity over a few days to a few weeks until conditions match those of the rest of the greenhouse.

Maintain optimal greenhouse conditions: Once acclimatized, ensure that the plants are kept in the following conditions:

• Light: Aim for a light intensity of about 10,000 to 15,000 lux.
• Temperature: Maintain a temperature range of 20-30°C (68-86°F).
• Humidity: Try to maintain a relative humidity of 50-70%.
• Watering: Water the plants when the top inch of soil becomes dry.
• Ventilation: Maintain good airflow to prevent disease but avoid causing plants to dry out too quickly.